Structural Basis of Cell Wall Cleavage by a Staphylococcal Autolysin
نویسندگان
چکیده
منابع مشابه
Structural Basis of Cell Wall Cleavage by a Staphylococcal Autolysin
The major autolysins (Atl) of Staphylococcus epidermidis and S. aureus play an important role in cell separation, and their mutants are also attenuated in virulence. Therefore, autolysins represent a promising target for the development of new types of antibiotics. Here, we report the high-resolution structure of the catalytically active amidase domain AmiE (amidase S. epidermidis) from the maj...
متن کاملActivity of the major staphylococcal autolysin Atl.
The major autolysin of Staphylococcus aureus (AtlA) and of Staphylococcus epidermidis (AtlE) are well-studied enzymes. Here we created an atlA deletion mutant in S. aureus that formed large cell clusters and was biofilm-negative. In electron micrographs, the mutant cells were distinguished by rough outer cell surface. The mutant could be complemented using the atlE gene from S. epidermidis. To ...
متن کاملOptical configuration of staphylococcal cell wall serine.
Studies on the amino acid content of the cell wall of gram-positive bacteria have shown them to be composed of relatively few amino acids. One of the principal amino acids found in the cell wall of certain staphylococci is serine (1, 2, 5, 6). Although the optical configuration of staphylococcal cell wall serine has not been determined, Whitney and Grula (8) found that Micrococcus lysodeikticus...
متن کاملStructural basis for sequence-dependent DNA cleavage by nonspecific endonucleases
Nonspecific endonucleases hydrolyze DNA without sequence specificity but with sequence preference, however the structural basis for cleavage preference remains elusive. We show here that the nonspecific endonuclease ColE7 cleaves DNA with a preference for making nicks after (at 3'O-side) thymine bases but the periplasmic nuclease Vvn cleaves DNA more evenly with little sequence preference. The ...
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ژورنال
عنوان ژورنال: PLoS Pathogens
سال: 2010
ISSN: 1553-7374
DOI: 10.1371/journal.ppat.1000807